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KMID : 0364820180540040410
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Korean Journal of Microbiology
2018 Volume.54 No. 4 p.410 ~ p.419
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Investigation of bacteria in the agricultural by-products imported for the use as media materials in mushroom cultivation
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Kim Jun-Young
Kim Su-San
Kim Seong-Hwan
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Abstract
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It is urgently required to construct safety data on agriculturalby-products imported for use as medium materials for domesticmushroom production. However, research on microorganismsis insufficient. This study was conducted to investigate thepresence of bacteria that have the possibility of harmful effectson human, plants and mushroom in wheat straw, peatmoss,cottonseed hull, cottonseed meal, and beet pulp imported fromAustralia, Canada, China, Egypt, Germany. Bacteria were foundin the range of 1.35 × 102to 8.34 × 106CFU/g. As a result of 16SrDNA sequence analysis, total of 19 genera and 45 species ofbacteria were identified. Bacillus genus was dominant, followedby Paenibacillus genus. At the species level, diverse species wasin the order of Firmicute, Proteobacteria and Actinobacteria. Regarding the agricultural by-products, straw and peat mosshad more diverse bacteria than other agricultural by-products. Among the indentified bacteria, 6 species of 5 genera (Enterobacterasburiae, Enterobacter ludwigii, Stenotrophomonas maltophilia,Pseudomonas monteilii, Bacillus anthracis, and Cellulosimicrobiumfunkei) were present as potent harmful bacteria to human. Surprisingly, both the human and plant pathogenic Klebsiellapneumoniae subsp. pneumonia was present. Bacillus altitudiniswas present as a plant pathogen. Lysinibacillus sphaericus,an insect pathogen, and Ochrobactrum pseudogrignonense, amushroom pathogen, were also present. The results of thisstudy confirmed that several kinds of pathogenic bacteria werepresent in the agricultural by-products for the mushroomcultivation medium imported into Korea. Our work suggeststhat hygiene inspection and management is urgently needed forimported agricultural by-products to be safely used for mushroomproduction.
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KEYWORD
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bacterial concentration and diversity, harmful bacteria, imported agricultural by-products, mushroom cultivation media materials
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